e0065 The association between the single nucleotide polymorphisms of matrix metalloproteinases and the carotid atherosclerosis in patients with essential hypertension

Objective To investigate the relationship between the single nucleotide polymorphisms (SNP) of matrix metallo proteases (MMP-2 -735C/T; MMP-3 -1171 5A/6A) and the carotid atherosclerosis (CAS) in Chinese Han and Uygur populations with EH. Methods The study comprised 276 Han nationality and 212 Uygur participants, who were divided into two groups: CAS (n=293) and NS (n=195). Genotypes were detected by PCR-RFLP and their frequencies were determined. Results (1) The frequencies of MMP-2 TT genotype and T allele in CAS were higher than in NS (Han : X 2 =11.441, p=0.003; Uygur : X 2 =28.255, p=0.000). In NS, the frequencies of TT genotype and T allele in Han were higher than in Uygur (X 2 =12.509, p=0.001)). (2) The frequencies of MMP−3 6A/6A genotype and 6A allele in CAS were higher than NS (Han: X 2 =7.523, p=0.024; Uygur: X 2 =6.474, p=0.039). The frequencies of MMP−3 6A/6A genotype and 6A allele in Han were higher than Uygur (CAS: X 2 =26.230, p=0.000; NS: X 2 =18.809, p=0.000). (3) The single gene analysis showed Han individuals with CT or TT genotypes had 2.25-fold risk and Han individuals with 6A/6A genotypes had 1.85-fold risk suffering from CAS. Han individuals with both T allele and 6A/6A genotypes had 3.17-fold risk suffering from CAS. The single gene analysis showed that Uygur individuals with CT or TT genotypes had 5.04-fold risk suffering from CAS. Uygur individuals with 6A/6A genotypes had 2.20-fold risk suffering from CAS. Uygur individuals with both T allele and 6A/6A genotypes had 3.20-fold risk suffering from CAS. (4)According to MMP-2 genetypes, Han individuals with MMP-2 CT+TT genotypes had higher LDL and lower HDL levels than CC genotype in CAS (LDL:2.9 mmol/l vs 2.6 mmol/l; HDL:1.2 mmol/l vs mmol/l). Uygur individuals with CT+TT genotypes had higher TG levels than CC genotype (CAS : 2.5 mmol/l vs 1.6 mmol/l; NS : 3.9 mmol/l vs 2.0 mmol/l). According to MMP−3, Han individuals with 6A/6A genotype had higher T-CHOL and LDL levels than 5A/5A+5A/6A genotypes in NS group (T-CHOL : 4.6 mmol/l vs 4.2 mmol/l; LDL: 2.3 mmol/l vs 2.2 mmol/l). (5) The binary logistic regression analysis showed MMP-2 CT+TT genetypes were the risk factors for CAS in individuals with EH (Uygur: OR=9.65; Han : OR=2.076). MMP-3 6A homogeneses were the risk factors for CAS in Han individuals with EH (OR=1.802). MMP-2 CT+TT and MMP-3 6A homogeneses had a combined influence on the incidence of CAS in Han individuals with EH. Conclusions (1) Han and Uygur individuals had differential distribution of MMPs. (2) The SNP of MMP-2 -735C/T is associated with CAS in individuals with EH. The MMP-2 T allele may be a risk factors on CAS in individuals with EH. The SNP of MMP-3 -1171 5A/6A is associated with CAS in Han individuals with EH. The 6A allele may be a risk factors on CAS in Han individuals with EH.