Determination of Nuclear Prostatic Androgen Receptors by FPLC (Fast Protein Liquid Chromatography)

The experimental detection of steroid receptors, quantitative as well as qualitative, has been received with wide-spread interest in the research of the various effects of steroid hormones during recent years. The quantitative measurement of cyto- and karyoplasmatic prostatic androgen receptors, carried out through such methods as the Dextran-Coated-Charcoal-Method (DCC) (Snochowsky et al. 1977), the gradient centrifugation method (Mainwaring and Milroy 1975; Menon et al. 1978) or the Hydroxyl-apatite process (Murthy et al. 1984; Pavlik and Coulson 1976), represent unprecise or complicated analytical processes, which are due to technical factors, such as proteolytical degradation, the time factor, temperature sensitivity, metabolic and biochemical reactions. Since the status of the estrogen receptors — especially regarding breast cancer — is currently a routine feature of therapeutic considerations, a practicable, valid check up process for hormonal therapeutic options as well as for the status of basic research relating to etiology and pathogenesis of benign and malignant prostatic changes appears to be a sensible project.