Effects of Pretreatment With Yisheng Injection on Renal Warm Ischemia/Reperfusion Injury in Mice

Abstract Introduction Due to the organ supply shortage, the donor pool has been expanded to include non–heart-beating donors, where renal warm ischemia/reperfusion (I/R) injury is inevitable. This study was undertaken to determine whether Yisheng injection (YM) could attenuate renal warm I/R injury in mice. Materials and Methods Male C57BL/6 mice were divided into sham, ischemic, and YM-treated groups using 50 minutes of left kidney ischemia. Mice were humanely killed at 4 or 24 hours postreperfusion. We assayed the effects of YM on liver functional injury, neutrophil recruitment, and proinflammatory mediators after renal I/R injury. Results Renal I/R produced dramatic injuries in mouse kidneys. Administration of YM reduced liver function (urea nitrogen of untreated vs treated, 4.7 ± 0.6 vs 26.6 ± 1.5 mmol/L; P P Conclusion YM protects murine kidneys from warm I/R injury, probably via decreasing functional injury, reducing neutrophil infiltration, and suppressing the overexpression of proinflammatory mediators and adhesion molecules.