Resequencing Pathogen Microarray (RPM) for prospective detection and identification of emergent pathogen strains and variants
2010
High-density resequencing microarrays support simultaneous detection and identification of multiple viral and bacterial
pathogens. Because detection and identification using RPM is based upon multiple specimen-specific target pathogen
gene sequences generated in the individual test, the test results enable both a differential diagnostic analysis and
epidemiological tracking of detected pathogen strains and variants from one specimen to the next. The RPM assay
enables detection and identification of pathogen sequences that share as little as 80% sequence similarity to prototype
target gene sequences represented as detector tiles on the array. This capability enables the RPM to detect and identify
previously unknown strains and variants of a detected pathogen, as in sentinel cases associated with an infectious disease
outbreak. We illustrate this capability using assay results from testing influenza A virus vaccines configured with strains
that were first defined years after the design of the RPM microarray. Results are also presented from RPM-Flu testing of
three specimens independently confirmed to the positive for the 2009 Novel H1N1 outbreak strain of influenza virus.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
1
Citations
NaN
KQI