Identification of an isolate of fowl adenovirus group I virus

An isolate of fowl adenovirus group I virus (FAVI-JS) was identified by virus morphology,cytopathogenic effect (CPE) and sequence analysis.The virus particles are non-enveloped,about 70 nm～80 nm in diameter,and have icosahedrad symmetry.it (can't) agglutinate red blood cells (RBC) of chicken,duck or goose,but can agglutinate rat cells.The virus caused CPE in chicken embryonic kindey (CEK) cells.L,R,ITR fragment of the viral genome were emplified by PCR.The sequences of these three fragments were analyzed by comparing with the relevant parts of CELO virus,FAV-1,FAV-A and FAV-8 obtained from GenBank.The results showed that the three fragments of these virus shared more than 96 %nucleotide sequence homology.In the phylogenetic tree inferred from ITR sequences,which are important in genetic to adenovirus,showed that FAV1-15 and fowl adenovirus group Ⅰ viruses converged into a lineage,especially closed to serotype I virus.The virus from group Ⅲ share the other lineage.From these results,we can confirmed that FAVI-JS is a member of groups I fowl adenovirus.