OPC-28326, a Selective Peripheral Vasodilator with Angiogenic Action, Attenuates Postinfarction Cardiac Remodeling

14 weeks old male C57Bl6 mice, total 6 mice were divided into two groups. After measuring blood pressures and body weight, we gave furosemide (100mg/kg) or tolvaptan (10mg/kg), respectively. These mice were kept in metabolic cages for 24 hrs, and then they were sacrificed to remove kidneys and blood.Experiments 2 Total 20 male C57Bl6 mice (10-12 weeks old) were divided into three groups with high sodium diet (8%NaCl), high sodium diet containing 0.05% furosemide, and high sodium diet containing 0.03% tolvaptan. Initially, 10 mice were kept in metabolic cages for 7 days and, subsequently, resting 10 mice were given both hydrochlorothiazide and amiloride for 5 days. Total RNA were examined by quantitative PCR methods and in situ hybridization for tubular renin were performed.Results: Enhanced renin expression were observed in the subjects with high sodium diet containing 0.05% furosemide. However, both thiazide and amiloride treatment significantly suppressed. The subjects with high sodium diet containing 0.03% tolvaptan did not show the enhanced renin expressions. Conclusions: Furosemide treatment enhanced renal renin gene expressions in both acutely and chronicly, however tolvaptan did not show the enhancement of renal renin gene expression. Therefore, tolvaptan treatment might be better for renal protection.