Abstract 627: Pro-Angiogenic Properties are Impaired in Bone Marrow (BM)-Derived Stem or Progenitor Cells in Diabetes and Diabetic Hindlimb Ischemia was Effectively Repaired by Non-Diabetic Stem or Progenitor Cells

Background: Recent studies reported that angiogenesis and endothelial progenitor cells (EPCs) were dysfunctional in diabetes (DM). However, there have been no investigations on the pro-angiogenic characteristics of BM-derived cells in diabetes. Accordingly, we investigated angiogenic defects in BM-derived mononuclear cells (BM-MNCs), EPCs and mesenchymal stem cells (MSCs) and sought to determine whether hindlimb ischemia can be repaired by non-DM cells. Method & Results: Studies were performed with C57Bl mice 3 months after the induction of diabetes with streptozotocin. Gene profiling analysis with Angiogenesis Microarray Kit (GEArray) and real-time RT-PCR showed that multiple angiogenic factors were significantly down-regulated in DM cells compared to non-DM control cells (Table 1). We also compared cell functions between DM and non-DM cells. DM-EPCs revealed decreased adhesion to laminin (non-DM vs DM, 78 vs 40/mm2, P<0.05) and vitronectin(320 vs 140/mm2, P<0.05), decreased migration to VEGF-A (210 vs 8...