Diminished Release of Atrial Natriuretic Factor in Calves with a Total Artificial Heart

: Venous hypertension after total artificial heart (TAH) replacement, a common problem in humans as well as experimental animals, is thought to be related to reduced release of atrial natriuretic kictor (ANF) due to atrial damage. To verify this hypothesis, we stimulated the release of ANF in 6 calves before (— 7 days) and after TAH replacement (+ 25. +50, +80. and + 100 days) by therapid(+= 10 min)infusion of 2 Lof Kinger's solution. In normal calves (-7 days) this procedure induced a rise in plasma concentration of ANF from 18.5 ± 12 to 31.6 ± 12 pmol/L (p < 0.05). After TAH the ANF release continuously decreased (analysis of variance. p < 0.02). Thus. ANF increased by 9.8 ± 9.3. 6.8 ± 17.5. 0.4 ± 5.2, and 0.5 ± 3.2 pmol/L at days 25. 50, 80, and 100, respectively. The central venous pressure increased by 3.4 ± 1.5 mm Hg (before TAH) and by 6 ± 2.9 mm Hg (after TAH) during this procedure. A decrease in plasma concentrations of aldosterone, most likely due to extracellular volume expansion and a decrease in total protein plasma concentrations and in hematocrit due to the dilution of blood, was seen in each experiment. Necropsy demonstrated massive atrial dilatation and myocairdial dystrophy with atrial fibrosis. ANF granules were present to a small extent in every calf. We conclude that rapid intravenous infusion is an adequate stimulus to release ANF in normal calves. AfterTAH implantation the release of ANF by high venous pressure declines although ANF granules are still present in the damaged atrial stumps. Therefore. the loss of stimulated ANF could contribute to the observed increase in venous pressure in TAH calves although other pathophysiologic mechanisms cannot be excluded.