A COMPARATIVE STUDY OF ANGIOTENSIN CONVERTING ENZYME (ACE) GENE POLYMORPHISM OF EASTERN INDIAN BENGALI AND WESTERN INDIAN MEWARI TYPE 2 DIABETES PATIENTS IN INDIA

Diabetes mellitus is a rapidly emerging global heal th problem and it is expected to cross the pandemic level within a span of another t wo decades. World-wide its frequency is expected to rise from 171 million to 366 million within the nex t two decades and India alone will take almost 25% shares from it to become the leading country with h ighest number of diabetic patients. For the present study 111 type 2 diabetes (59 Bangali and 52 Mewari) were enrolled. Anthropometric measurement and Clinical data- including information on duratio n of diabetes, presence of any complication, histor y of other disorders, systolic and diastolic blood pr essure- were collected. The weight (kg) and height (cm) were recorded, the body mass index (BMI) was calculated and Genomic DNA was isolated from 5ml of peripheral blood samples of 111 type 2 diabe tes individuals. Further, PCR has been performed to know the frequency of ACE I/D polymorphism. Our study reveals that anthropometric measurements and few bio-chemical parameters were significantly varying in type 2 diabetes patients among western Indian Mewari population and eastern Indian Bengali population. Distribution of ACE genotypes as well as their allele frequency doesn't differ significantly. It can presume that ACE gene I/D polymorphism, an Alu insertion polymorphism, can be used as a suitable marker for studying genetic variation among different human populations because of its stable nature as well as representing a unique evolutionary event.