Correlation of clinical data and expression levels of six tumor supressor genes in breast cancer.

CTRC-AACR San Antonio Breast Cancer Symposium: 2008 Abstracts Abstract #1058 As the scientists and physicians began to share more information for improving the personalization in medicine, we aimed to check the expression profiles for major tumor suppressor genes of 70 breast tumor samples obtained from the Dokuz Eylul Breast Tumor Biobank. We tried to figure out the levels of expressions of tumor suppressor genes by the quantity of the RNA which belongs to the examined gene in the tumor tissue samples. The expression level was measured by quantitative RT-PCR using total RNA extracted from the tissues. Fluorescence amplification plots were observed and the Ct values were used for comparing the expression levels. For quantification, we chose to normalize the Ct differences by using Genorm software with averaging four housekeeping genes (PUM1, RPL13A, ACTB, B2M). Correlation analysis is performed between genetic expression levels and the data obtained from the clinical records of the biobank. Although further statistical analysis is still continuing, we observed direct significant correlations between ki67 level and BRCA1 expression and between intraductal component level and CDH1 expression. Also significant relations between tubuler formation and BRCA1 expression; between intraductal component and P16 expression were observed. Dermal infiltration had significant correlatios with both TIMP3 and P16 expressions. Significant inverse correlation between estrogen receptor level and BRCA1 expression were found as well as the inverse correlation between progesteron receptor level and TIMP3 and BRCA1 expression levels. Staging had only significant correlations with TIMP3 levels. We also found out that expression status of BRCA1 and BRCA2; BRCA2 and CDH1; BRCA1 and GSTP1, and GSTP1 and TIMP3 shows significant correlations within themselves. *This study is supported by TUBITAK, The Scientific and Technological Research Council of Turkiye. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 1058.