A novel method to reduce prolonged warm ischemia injury in amputated limbs

INTRODUCTION: In the US it is estimated that more than 1.2 million partial or complete limb amputations per year cannot be saved because of excessive ischemia time. The purpose of this study was to determine whether perfusion with a novel fusogenic lipid vesicle (FLV) that delivers magnesium adenosine triphosphate (Mg-ATP) directly into cells could maintain amputated limbs viable after prolonged warm ischemia.