Antioxidant M odulating effect of Olea europeaea leaf E xtract on Superoxide dismutase (SOD) A ctivity in Streptozotocin induced diabetes

Oxidative stress concern one of the most common causes of chronic diseases worldwide. Earli er clinical studies reported that oxidative stress plays a major role in the pathogenesis and development of complications of both types of diabetes mellitus (DM). Most of the synthetic diabetic drugs failed to minimize the side effects. Hence, now a days natural antioxidants gaining popularity in the treatment of diabetes to overcome the adverse effects of synthetic diabetic drugs. The purpose of the current work was to investigate the modulating effect of Olea europeaea leaf extract on antioxidativ e enzyme (Superoxide dismutase) activity in Streptozotocin ( STZ ) induced diabetes . Oral administration of Olea europaea methanolic leaf extract (OEMLE) at the doses 250, 500 mg/kg, p.o respectively was studied in STZ induced diabetic mice. Oral glucose tolerance, blood glucose level as well as liver and kidney and serum superoxide dismutase activit ies were studied. After treatment with OEMLE 250, 500 mg/kg, p.o for 15 days there was a significant decrease in blood glucose levels and Oral glucose tolerance test reviled maximum blood glucose levels after oral glucose challenge after 30 min. The activity of SOD in serum, liver and kidney activity was significantly increase (p < 0.05 ) after the oral treatment with OEMLE (250, 500 mg/ kg b.w) . We concluded that OEMLE (250, 500 mg/kg, b.w) doses are effective in the protection of oxidative damage in diabetes. The effectiveness is does dependent manner. Our study suggest that OEMLE 500mg/kg , b.w was more effective dose. Keyw ords: Oxidative stress , Superoxide dismutase , Olea europeaea leaf , diabetes mellitus .