Morphological and optical properties of human immature platelet-enriched population produced in immunodeficient mice

AbstractUltrastructure analysis of immature platelets is difficult because of the lack of a suitable marker and their relatively low concentration in total platelets.We investigated the morphological and optical properties of human immature platelets produced and enriched in immunodeficient mice via human CD34-positive cell administration.Immunodeficient mice were injected with human CD34-positive cells and administered eltrombopag orally for 14 days (eltro-mice). Some of these mice were maintained for 2–3 months (steady-state-mice). Platelets were double-stained with a human CD41 antibody and a nuclear staining dye (Sysmex hematology analyzer XN series reagent), and then analyzed by flowcytometry FCM to identify human immature platelets. Human CD41-positive cells were isolated from citrated blood by magnetic cell sorting with human CD41 antibody, and examined using electron microscopy.Flow cytometric analysis with the XN reagent demonstrated that peripheral blood from eltro-mice had a higher percentage o...