Platelets of type 2 diabetic patients are characterized by high ATP content and low mitochondrial membrane potential

Platelet dysfunction plays a critical role in vascular complications of type 2 diabetes mellitus (T2DM). But the relationship between platelet hyperactivity and its energy metabolic process remains unclear. This study was designed to explore alterations of platelet mitochondrial ATP production and the possible mechanism. A total of 39 T2DM patients without macrovascular and microvascular complications and 32 normal controls were fasting sampled. Platelet ATP content was measured by a high performance liquid chromatograph (HPLC). The flow cytometry technique was adopted to evaluate mitochondrial membrane potential (ΔΨm), the stored force for platelet ATP production. Consequently, T2DM patients exhibited obvious hyperglycemia, hyperlipidemia and hypertension, but normal platelet morphology. Platelet ATP content was significantly higher in T2DM (0.032 ± 0.010 µmol/109 platelets versus 0.017 ± 0.006 µmol/109 platelets, p < 0.001) than in the control group. Interestingly, ΔΨm was markedly decreased in T2DM pat...