Flow cytometric assay of platelet glycoprotein receptor numbers in hypercholesterolemia

In this study, platelet glycoprotein (Gp) receptor numbers were measured by a flow cytometric assay using Cytoquant Gp in seven hypercholesterolemic and five normal subjects. Thrombin receptor agonist peptide (TRAP) was used to activate platelets. In hypercholesterolemia the Gp receptor numbers per resting platelet were found to be: 38 629 - 8538 (GpIIb/IIIa), 22 269 - 5628 (GpIb), 37 037 - 9810 (GpIIIa), 224 - 504 (CD62-P). After activation, receptor numbers were determined to be: 56 399 - 9003 (GpIIb/IIIa), 10 970 - 5319 (GpIb), 50 715 - 7904 (GpIIIa), 1222 - 687 (P-selectin). In the normal group before the activation, receptor numbers were: 43 828 - 8862 (GpIIb/IIIa), 22 166 - 3847 (GpIb), 42 351 - 1049 (GpIIIa), 62 - 139 (CD62-P), After activation, receptor numbers were determined to be: 60 573 - 4294 (GpIIb/IIIa), 13 003 - 4118 (GpIb), 52 067 - 1039 (GpIIIa), 3608 - 1508 (CD62-P). In hypercholesterolemic subjects, GpIIb/IIIa and GpIIIa receptor numbers on activated platelets increased significantly, ...