Gastric Perfusion of Vitamin D_3 with Different Diets Induce the Atherosclerotic Rat Model

Objective To investigate the optimun approach to induce the atherosclerotic rat model.Methods Three methods were used to induce atherosclerotic model,including: simple high fat diet feeding,high fat diet feeding with gastric perfusion of Vitamin D3,and gastric perfusion of Vitamin D3 with common rat food(splashed oil on the normal rat food with yolks and peanuts).Histopathology change of the atherosclerotic plaques in aorta was observed by normal and histopathologic sections and Hematoxylin staining.Results The endarterium of aorta of the rats with simple high fat diet was smooth without atherosclerotic plaques after 2 months.2 rats with high fat diet feeding and gastric perfusion of Vitamin D3 had formed miliary atherosclerotic plaques.In the rats with vitamin D3 and common rat food,the ring-shape calcified atherosclerotic plaques were found in the all endarterium of aorta.Conclusion Gastric perfusion of Vitamin D3 once a week(3 times) combined with common rat diet could induce the atherosclerotic model successfully and decrease the mortality rate.