Melatonin modulates adiponectin expression on murine colitis with sleep deprivation

Core tip: We report this first study that melatonin and sleep deprivation are related to adiponectin expression in the colonic mucosa of murine colitis. C57BL/6 mice were feeding with 2% DSS for inducing colitis and using specially designed multiple platform water baths for sleep deprivation. Immuno-histochemical staining and Western blot analysis was performed using anti-adiponectin antibody. The expression level of adiponectin in mucosa was decreased in colitis, with the lowest level observed in colitis combined with sleep deprivation. Melatonin injection significantly (P < 0.05) recovered the expression of adiponectin. This study suggests that melatonin and adiponectin synergistically potentiate the anti-inflammatory effects in murine colitis.