Pharmacodynamic studies of cyclosporine in marrow transplant recipients: A comparison of three assay methods

We investigated the correlation between trough cyclosporine concentration in plasma measured by polyclonal fluorescence polarization immunoassay (FPIA) and polyclonal radioimmunoassay (RIA) or in whole blood measured by high-performance liquid chromatography (HPLC) and the risk of renal dysfunction or acute graft-versus-host disease in 29 patients undergoing allogeneic bone marrow transplantation for leukemia. The FPIA and RIA values were highly correlated (r=0.93) and on the average CsA concentrations measured by FPIA were 1.56 times higher than those measured by RIA