Optimization of nano spray drying parameters for production of α-amylase nanopowder for biotheraputic applications using factorial design

AbstractThis study was designed to optimize the effect of operating conditions and formulation parameters using various additives to develop α-amylase nanoparticles. α-Amylase was chosen due to its importance in the substantial number of industrial processing with emphasis on pharmaceutical industry. Factorial statistical design was adopted to effectively optimize the size, yield value, residual enzyme activity, and morphology of α-amylase nanoparticles using Nano Spray Dryer BUCHI B-90. The physicochemical characterization of the prepared nanopowder was carried out using zetasizer and scanning electron microscopy (SEM) and enzyme activity assay. Results showed that the type of additive and mesh size significantly influenced the particles size and yield value. SEM images showed three different structure patterns where particle morphology was influenced by Tween® 80 or sucrose at low concentration (0.05%). Optimized spherical nanoparticles (600 nm) was obtained using 7 µm mesh cap size, sucrose (0.15%), 95...