Abstract 19259: Dynamic Metabolomic Profiling Reveals Evidence for Metabolic Inflexibility in the Failing Heart

Introduction: Metabolic signatures of heart failure (HF) phenotypes generally portray static, single time point differences between disease and control groups. Dynamic changes in metabolite in response to cues such as glucose loading are rarely determined in human HF studies. Methods: This was a multi-centre study that involved HF patients with reduced ejection fraction (EF) and controls. All patients underwent a protocol that involved fasting overnight, followed by an early morning oral glucose drink (75 gram). Blood sampling at (Time 1) baseline (prior to glucose drink), (ii) (Time 2) 2 hours after glucose drink, (iii) (Time 3) 3 hours after baseline were performed. Targeted high mass spectrometry measured 65 circulating acyl-carnitines in serum (nanomolar). Results: This analysis consisted of 19 subjects (mean age 53±10 years; 8 females (42%) (HF n=9 (all EF <50%); controls n=10). All subjects were non-diabetic, as confirmed by fasting glucose and glycated haemoglobin levels. At Time 1, there were sign...