Association of post-stroke depression with tryptophan hydroxylase 2 rs4641528 polymorphism and risk factors of post-stroke depression

Objective To investigate the association of post-stroke depression (PSD) with tryptophan hydroxylase 2 (TPH2) gene polymorphism and related clinical risk factors of PSD. Methods Three hundred and seventy-six patients diagnosed as having acute ischemic stroke for the first time, admitted to our hospital from March 2015 to September 2017, were chosen in our study. According to Hamilton depression scale scores and Diagnostic and statisistical Manual of Mental Disorders, fourth edition (DSM-IV), these patients were divided into PSD group and non-PSD group. High-resolution melt analysis was used to complete the genotyping of TPH2 gene rs4641528 and rs1386494 in the enrolled patients. The correlations of single nucleotide polymorphisms and PSD were analyzed. Logistic regression analysis was performed on items with statistically significant differences in univariate analysis to identify independent factors affecting PSD. Results There were 104 patients into the PSD group and 272 patients into the non-PSD group; there were statistically significant differences between the two groups in years of education and NIHSS scores (P 0.05); there were significant differences in rs4641528 genotype and allele frequency between the two groups (P> 0.05). The C allele in the chromosomes of PSD patients accounted for 54.3%, while the C allele in the chromosomes of non-PSD patients accounted for 43.4%; the presence of allele (C) increased the risk of PSD (OR=1.552, 95%CI: 1.126-2.141, P=0.007). The results of multivariate Logistic regression analysis showed that baseline NIHSS scores and genotypes of rs4641528 (C/C+C/T) were independent influencing factors of PSD. Conclusion TPH2 rs4641528 gene polymorphism and baseline NIHSS scores are found to be associated with depression 3 months after stroke in south Anhui province. Key words: Stroke; Post-stroke depression; Tryptophan kinase 2; Gene polymorphism