Development of clay-protein based composite nanoparticles modified single-used sensor platform for electrochemical cytosensing application

Abstract A novel sensor platform modified with clay-protein based composite nanoparticles (Mt-HSA NCs) was developed to be used in electrochemical cytosensing application for the first time. The nanocomposite synthesized with desolvation method was structurally clarified by various characterization methods. Then, the working electrode was constructed by modifying the surface of the disposable pencil graphite (PGE) with physical adsorption to perform a simple sensor system. The characterization studies proved that the Mt-HSA NCs modified surface had a biocompatible, hydrophilic and large surface area where cancer cells can easily attach to the surface. As a diagnostic method, electrochemical impedance spectroscopy (EIS), which has become very popular in recent years, was carried out. The linearity range was found as from 1.5 × 10 2 to 7.5 × 10 6 breast cancer (MCF-7) cells and the limit of detection was calculated as 148 cells mL −1 . From these results, a simple, effortless, cost effective and rapid electrochemical impedimetric sensor system for the diagnosis of breast cancer was developed by examining the interaction of Mt-HSA NCs/PGE surface with MCF-7 cells.