AUTOLOGOUS SKELETAL-MYOBLAST-SHEET TRANSPLANTATION IMPROVED PORCINE CARDIAC FUNCTION WITHOUT INCREASING RISK OF ARRHYTHMIA

Methods: Male mini-pigs underwent LAD occlusion by a balloon catheter for 2 h, followed by reperfusion. After 4 weeks, 12 SkM sheets were implanted onto infarcted myocardium (Sheet-group n = 8), the same amount of cells were injected into the myocardium (Injection-group n = 7) and sham operations were performed (Sham-group n = 7). Implantable ECG loop recorder (ILR) was implanted subcutaneous on the left thorax. At 4 weeks after the transplantation, we assessed cardiac function with MDCT, interrogated ILR, and performed programmed ventricular stimulation (PVS). For assessing the inflammatory and injury response, inflammation factors, and high-sensitive CRP and Troponin-I were measured at 1, 3, 7, and 30 days after the transplantation by cytokine array method and ELISA, respectively. For observing inflammation, the tissues were stained immunochemically with antimacrophage.