Effects of Total Flavonoids of Sophorae Flos on the Levels of Insulin, Leptin and C-Peptide in Serum in Diabete Rat Model

OBJECTIVE To investigate the effect of total flavonoids of Sophorae Flos (TFSF) on the levels of insulin in serum, leptin and C-peptide in diabete rats which was established by streptozotocin. METHODS The diabete rat model was established by injected with 60 mg·kg 1 streptozotocin, and then randomized into 5 groups: large dosage TFSF group(600 mg·kg 1 ), middle dosage TFSF group(300 mg·kg 1 ), small dosage TFSF group(150 mg·kg 1 ), metformin group(208 mg·kg 1 ), and model group. Control group was set up with healthy normal rats. All the rats were administrated once a day for 30 days. Blood glucose in the limosis were measured on the 20, 30 day and then rats were sacrificed. The levels of insulin, insulin antibody, leptin, C-peptide in orbital blood were measured. RESULTS Compared with control group, the level of blood sugar and leptin in model group increased obviously, the level of insulin and C-peptide decreased obviously. It illustrated that the diabete model was established successfully. Compared with model group, the level of blood sugar in TFSF groups obviously decreased after 20 or 30 days, the level of insulin and C-peptide increased obviously after 30 days, while the level of leptin decreased obviously. CONCLUSION TFSF has good hypoglycemic effect on the diabete rats established by injection of streptozotocin. It can decrease the level of blood sugar and leptin, and increase the level of insulin and C-peptide significantly.