Reproducibility of p53 Immunohistochemistry in Bladder Tumors
2000
The
National Cancer Institute Bladder Tumor Marker Network conducted a
study to evaluate the reproducibility of immunohistochemistry for
measuring p53 expression in bladder tumors. Fifty paraffin blocks (10
from each of the five network institutions) were chosen at random from
among high-grade invasive primary bladder tumors. Two sections from
each block were sent to each laboratory for staining and scoring, and
then all sections were randomly redistributed among the laboratories
for a second scoring. Intra- and interlaboratory reproducibility was
assessed with regard to both staining and scoring. For overall
assessments of p53 positivity, the results demonstrated that
intralaboratory reproducibility was quite good. Concordance across the
five participating laboratories was high for specimens exhibiting no or
minimal nuclear immunostaining of tumor cells or high percentages of
tumor cells with nuclear immunoreactivities. However, there was a
reduced level of concordance on specimens with percentages of stained
tumor cells in an intermediate range. The discordancies were due mainly
to staining differences in one of the five laboratories and scoring
differences in another laboratory. These results indicate that some
caution must be used in comparing results across studies from different
groups. Standardization of staining protocols and selection of a
uniform threshold for binary interpretation of results may improve
assay reproducibility between laboratories.
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