Characterization of glucose transport by cultured chick pigmented epithelium

Abstract Glucose transport was studied in well-differentiated, confluent cultures of chick pigmented epithelial cells. Glucose permeation was stereospecific and was not influenced by energy poisons. Glucose, 3- O -methyl-glucose, and 2-deoxy- d -glucose all appeared to use the same carrier system. Countertransport of 3- O -methyl-glucose was demonstrated. The K m and V max values for 2-deoxy- d -glucose uptake were 2·70 m m and 22 nmol/min/mg protein. Hexose transport in these cultured cells appears to take place by a facilitated diffusion system.