Effect of serum from Kawasaki disease children with coronary artery lesions on expressions of NF-κB and matrix metalloproteinase-9 in human umbilical vein endothelial cells

Objective To investigate the effect of serum from Kawasaki disease(KD)children with coronary artery lesions on the expressions of NF-κB and matrix metalloproteinase-9(MMP-9)in human umbilical vein endothelial cells(HUVECs).Methods According to different culture medium compositions,the cultured HUVECs were divided into 6groups of C(normal serum),F(common fever serum),Non-CALs(serum from KD children without coronary artery lesions),CALs(serum from KD children with coronary artery lesions),PDTC(an inhibitor of NF-κB)+ Non-CALs and PDTC+CALs.After cultured for three hours,the expressions of NF-κB in HUVECs and MMP-9in supernatant were detected by Western blot and ELISA.Results Compared with groups of C and F,the expressions of NF-κB in HUVECs and MMP-9in supernatant were increased in groups of Non-CALs and CALs(P0.01),which were higher in group CALs than those in group Non-CALs(P0.01).Compared with groups of Non-CALs and CALs,the expressions of NF-κB in HUVECs and MMP-9in supernatant were decreased in groups of PDTC+ Non-CALs and PDTC+CALs(P0.01).Conclusion The upregulation of NF-κB and MMP-9 may play important roles in the formation of coronary artery lesions of KD children.