False-Positive Flow CytometryCrossmatch Results With Pronase-Treated T Cells in Human Immunodeficiency Virus-Infected Patients

A positive flow cytometrycrossmatch (FCXM) is often a contraindication for transplantation. Nonspecific binding of IgG antibodies to Fc receptors on B cells decreases sensitivity and specificity of the B-cell crossmatch. To prevent this, pronase treatment (PT) of lymphocytes is widely used. Effects of PT on T-cell FCXM are not well documented. We have observed a high positive T-cell FCXM …