Highly Selective and Repeatable Surface-Enhanced Resonance Raman Scattering Detection for Epinephrine in Serum Based on Interface Self-Assembled 2D Nanoparticles Arrays

Target analyte detection in complex systems with high selectivity and repeatability is crucial to analytical technology and science. Here we present a two-dimensional (2D) surface-enhanced resonance Raman scattering (SERRS) platform, which takes advantages of the high selectivity of the SERRS sensor as well as the sensitivity and reproducibility of the interfacial SERS platform, for detecting trace epinephrine (EP) in the serum. To realize sensitive and selective detection of EP in a complex system, Au NPs are modified with α,β-nitriloacetic acid and Fe(NO3)3 to form the Au NP-(Fe-NTA) sensor, and as a consequence, EP can be rapidly captured by the sensor on the surface of Au NPs and then delivered at the cyclohexane/water interface. More importantly, we synthesized the extremely stable Au NPs (PVP-stabilized Au NPs), where the presence of PVP prevents aggregation of Au NPs during the self-assembly process and then makes a more uniform distribution of Au NPs with analytes at the cyclohexane/water interfac...