DNA sequence analysis of long PCR amplified products at the D1S80 locus

Abstract Several PCR amplified products of larger alleles than 41 were observed frequently in D1S80 typing. These long alleles have rarely been analyzed. A total of seven amplified products of larger alleles than 41 at D1S80 were selected for sequence analysis. From the results, all of the samples have a repeat of only 16 base pairs in size, without the first repeat unit. In this study, the largest allele was determined to be the allele 72.