The correlation between level of serum APPL1 and urinary albumin excretion rate in patients with type 2 diabetes mellitus

Objective To investigate the association of adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1(APPL1)with urinary albumin excretion rate in patients with type 2 diabetes mellitus(T2DM), and to explore the role of APPL1 in the development of diabetic kidney disease(DKD). Methods According to the urinary albumin/creatinine ratio(UACR), 288 newly-diagnozed patients with T2DM were divided into normal albuminuria group(UACR 300 mg/g, n=77). 130 healthy subjects with matched sex and age were used as control group. Serum APPL1, tumor necrosis factor α(TNF-α), and adiponectin levels were measured by ELISA method. Results Serum APPL1 level in T2DM patients was significantly higher than that in control subjects(P<0.01), and increased with the rising of UACR. In patients with T2DM, serum APPL1 level was negatively correlated with estimated glomerular filtration rate(r=-0.246, P<0.01)while it was positively correlated with HbA1C, low density lipoprotein cholesterol, total cholesterol, triglycerides, insulin resistance index, serum creatinine, blood urea nitrogen, systolic blood pressure, TNF-α, and adiponectin(r=0.119, 0.167, 0.209, 0.194, 0.273, 0.242, 0.131, 0.144, 0.365, and 0.952, respectively, P<0.05 or P<0.01). Conclusion Serum APPL1 level in patients with T2DM was increased with the rising of UACR, suggesting that APPL1 may be involved in the development of DKD. (Chin J Endocrinol Metab, 2017, 33: 833-837) Key words: Diabetes mellitus, type 2; Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1; Diabetic kidney disease; Urinary albumin excretion ratio