P2 Tissue factor pathway inhibitor (TFPI) in normal and complicated pregnancy

s selected for Poster Presentation P1 Reduced fibrinolytic activity in women after delivery in Benin City, Nigeria J.O. Osakue, O.I. Ajayi, A. Ande. Department of Physiology and Obstetrics/Gynecology, University of Benin and University of Benin Teaching Hospital, Nigeria Abstract: Fibrinolytic activity (using ELT) and plasma fibrinogen concentration in 50 mothers just after delivery were assessed (giving 50 mothers and 50 cord samples). Also 50 healthy women without delivery were used as controls. All these were to ascertain if there is any change in fibrinolytic activity in women after delivery. Methodology: Fifty women (22 35 years) with normal delivery were used for this study (150 samples). Blood samples were collected and investigations were performed before noon. ELT (fibrinolytic activity) was carried out using the method of Cecil Hougie (1986) and plasma fibrinogen concentration (PFC) was estimated using Ingram clot weight method (1961). The t-distribution test was used to test for level of significance between mean of both test and controls, P< 0.05 was significant. Results: The results show significantly lower fibrinolytic activity in mothers and significantly higher fibrinogen concentration than controls (P< 0.05). Maternal PFC and ELT values were also significantly higher than cord samples. Conclusion: There is reduced fibrinolytic activity and increased fibrinogen concentration in mothers immediately after delivery. This may be a proctective coagulative mechanism against excessive post partum haemorhage. Fibrinolytic activity (using ELT) and plasma fibrinogen concentration in 50 mothers just after delivery were assessed (giving 50 mothers and 50 cord samples). Also 50 healthy women without delivery were used as controls. All these were to ascertain if there is any change in fibrinolytic activity in women after delivery. Methodology: Fifty women (22 35 years) with normal delivery were used for this study (150 samples). Blood samples were collected and investigations were performed before noon. ELT (fibrinolytic activity) was carried out using the method of Cecil Hougie (1986) and plasma fibrinogen concentration (PFC) was estimated using Ingram clot weight method (1961). The t-distribution test was used to test for level of significance between mean of both test and controls, P< 0.05 was significant. Results: The results show significantly lower fibrinolytic activity in mothers and significantly higher fibrinogen concentration than controls (P< 0.05). Maternal PFC and ELT values were also significantly higher than cord samples. Conclusion: There is reduced fibrinolytic activity and increased fibrinogen concentration in mothers immediately after delivery. This may be a proctective coagulative mechanism against excessive post partum haemorhage. P2 Tissue factor pathway inhibitor (TFPI) in normal and complicated pregnancy A. Ittel1, F. Bretelle2, M.A. Coulomb2, L. Boubli2, G. Sebahoun1, D. Arnoux1. Departments of 1Hematology and 2Obstetrics, CHU Nord, Marseille, France TFPI plays a crucial role in haemostatic balance by regulating the initial step of blood coagulation. Since it is expressed by both endothelial and trophoblastic cells; TFPI could be of particular importance at the placental level. In this study, we measured free TFPI antigen (elisa method; Diagnostica Stago) in 4 groups of women: (1) 35 healthy nonpregnant women with no oral contraception and no history of pregnancy complications; (2) 47 women at the beginning (<12 weeks) and (3) 103 women at the end of a normal pregnancy; (4) women with late pregnancy complications (IUFD, IUFGR, PE) at the time of obstetrical event (n = 75) and/or at distance of pregnancy (n = 66). In normal pregnancy, mean TFPI level at delivery was significantly higher than in the first trimester (7.5 vs 5.2 ng /ml; p < 0.001) but lower than in non-pregnant controls (9.1 ng /ml; p = 0.002). In patients, TFPI levels varied widely at the time of the adverse event but were found significantly lower than in non-pregnant controls when measured at distance of pregnancy. (mean 6.6 vs 9.1 ng/ml, p < 0.001), TFPI level being below the lowest value of controls in 23% of patients. Among other pro-thrombotic abnormalities, low TFPI levels could play a role in the occurrence of placental vascular complications. P3 A score based prevention strategy of venous thromboembolism in pregnant women Y. Dargaud1, L. Rugeri1, M.C. Vergnes2, B. Arnutti3, P. Amaranto4, C. Negrier1, J. Ninet5, P. Gaucherand6, M.C. Trzeciak1. 1Unite d’Hemostase Clinique, CHU de Lyon, France, 2Laboratoire d’Hemostase, CHU de Bordeaux, France, 3Service d’Hemostase et d’Hemovigilance, CH de Valence, France, 4Service de Medecine Interne, CH de Roanne, France, 5Service de Medecine Interne, CHU de Lyon, France, 6Service de Gynecologie Obstetrique, CHU de Lyon, France Objective: To evaluate the usefulness of score based management of pregnancies with high risk of venous thromboembolism (VTE). Table 1. VTE risk prediction score