Lipid peroxidation and genetic polymorphisms of enzyme Glutathione-S-Transferase in hemodialysis patients

Background Lipid peroxidation changes in some diseases. Glutathione-S-transferase (GST) detoxifies reactive electrophiles and products of oxidative stress. The aim of present study was to assess the lipid peroxidation and genetic polymorphisms of glutathione S -transferase in hemodialysis patients. Materials and methods The study included 45 control groups and 45 hemodialysis patients. Glutathione S -transferase genotyping was done by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism technique. Results The frequency of GSTM1+ genotype was lower in the hemodialysis patients than in the control groups (P=0.001). The mean MDA was high in all GST genotypes in the hemodialysis patients ( P < 0.001). The GST activity was low in the GSTT1- , GSTT1+ , GSTM1- , GSTM1+ , GSTT1 / GSTM1, GSTT1 / 0 and 0/ GSTM1 genotypes in the hemodialysis patients ( P < 0.001). There were positive correlation between MDA and GSTM1 (P=0.001) and GSTT1 (P=0.022). GSTM1 with GST and MDA with GST showed negative correlation (P=0.001). Conclusion These findings showed that GSTM1- may be an important risk factor for developing end stage renal disease. Our results indicated that there is a significantly increased MDA level among GSTM1-/GSTT1- genotypes in all study groups compared with GSTM1+/GSTT1+ genotypes. These findings suggest that for estimation of vulnerability to chronic renal failure, the GST gene polymorphism may use as a suitable genetic marker.