Effect of Ginaton combined calcium inhibitor on apoptosis of myocardial cell of coronary heart disease in rats after liquor poisoning

Objective To observe the effect of Ginaton combined calcium inhibitor on apoptosis of myocardial cell of coronary heart disease in rats after liquor poisoning.Methods 80 rats with coronary heart disease after liquor poisoning were divided into four groups: Each group divided into four time points.According to different group injected different drug and got samples after treatment at different time.The expression of the Bc1-2 and Caspase-3 were examined by immunohistochemistry techniques and the apoptosis of myocardial cell were examined by TUNEL method.Results In the model group,levels of Bc1-2 expression decreased,but levels of Caspase-3 expression increased.Comparing with model group,levels of Bc1-2 expression increased,levels of Caspase-3 expression decreased in Ginaton treatment group and Nimodipine treatment group(P0.05);Comparing with Ginaton treatment and Nimodipine treatment,levels of Bc1-2 expression obviously increased,levels of Caspase-3 expression obviously decreased in Ginaton combined Nimodipine treatment(P0.01).Conclusion Ginaton combined calcium inhibitor can significantly increase levels of Bc1-2 of rats with coronary heart disease after liquor poisoning,suppress levels of Caspase-3,decrease cell population of apoptosis,suppress of apoptosis myocardial cell.Ginaton combined calcium inhibitor treat coronary heart disease in rats after liquor may through suppress of apoptosis myocardial cell.