Comparison of Fed-batch Strategies in Hybridoma Cultures

The classical cell culture process in stirred tank or airlift reactors is still the mostly used large scale production system. Fed-batch processes with different feeding strategies have been investigated to improve the productivity of hybridoma cultures. The following approaches for substrate control are compared in this work: model-based control, online characterisation and the application of linear feed trajectories. The required knowledge and the effort for implementation is discussed.