Neonatal maternal separation results in stress-induced visceral hyperalgesia in adult rats: A new model for IBS

Visceral hyperalgesia and allodynia, somatic normolhypoalgesia, and autonomic dysregulation of the gut are the hallmarks of irritable bowel syndrome (lBS). The pathophysiology of IBS remains unclear, due in large part to the lack of an analogous animal model. However, it has been the MMC cycle (first MMC 104.11 (6.9) min, second MMC 114.7 (8.6)min) was observed. Nor did bile infusion have an effect on the start of the next phase III (90.9 (8.6) min vs 75.7 (6.8) min, p=ns). Bile infusion did not affect the origin of the phase III (antral:duodenaI4:3 after bile, 5:2 after saline). 10 em distal to the infusion port (03) a significant higher frequency of contractions (1.1 (0.7) vs 0.5 (0.8), p=O.012) and a higher motility index (6.8 (l) vs 4.4 (1.3), p=0.OO7) was observed after bile infusion. This effect was not present at 20 em (04). No increase in motor activity was observed after saline infusion. There was no significant variation in CCK plasma levels before vs after bile infusion (0.25 (0.24) vs 0.62 (0.5) pmol/l). Conclusion: The increase in motor activity observed after infusion seems to be a local effect of exposition to bile of the duodenal wall. Infusion of bile did not affect the timing of the MMC. It is therefore unlikely that intraduodenal bile plays a mayor role in the initiation of phase III of the MMC.