Biosynthesis of silver nanoparticle by Erwinia carotovora for Nanobiosensor fabrication

The development of reliable technology for the biosynthesis of silver nano particle using microorganism is an important aspect in the current scenario. The study was conducted at the Department of Agricultural Microbiology, Tamil Nadu Agricultural University. In this study the silver nitrate was used as the substrate for the biosynthesis of silver nanoparticle using Erwinia carotovora soft rot inducing pathogen in banana. The test strain was cultivated in nutrient broth for a period of 24 h. Different concentrations of silver nitrate were added to the supernatant of the test culture and 10 mM concentration was found optimum for the biosynthesis of silver nanoparticle. The silver nanoparticle was characterized by UV Visible spectroscopy and scanning electron microscope. The characterization with UV visible spectroscopy was found to be a useful technique for the analysis of nanoparticle, the broad plasmon peak at 390 and 410 nm was observed for the silver nanoparticle synthesized using Erwinia carotovora. SEM micrograph recorded a particle size of 50 – 100 nm. The silver nanoparticle synthesized was further utilized for the fabrication of nanobiosensor for the detection of Erwinia carotovora.