Histomorphometric evaluation of the rat kidney submitted to warm ischemia and the protective effect of resveratrol

Abstract Background The objective of this study is to quantitatively evaluate the protective effects of resveratrol for using during renal warm ischemia. Methods Rats were allocated into 4 groups: Sham, Sham Resveratrol, Ischemia, Ischemia Resveratrol. Sham Resveratrol and Ischemia Resveratrol received resveratrol before surgery. Ischemia and Ischemia Resveratrol had renal vessels clamped. Animals were euthanized four weeks after. Serum urea and creatinine were measured. Renal weight and volume, cortex-non-cortex areas ratio, cortical volume, glomerular volumetric density, volume-weighted mean glomerular volume and number of glomeruli per kidney were evaluated. Results Serum urea in Ischemia increased by 10.4% compared to Sham and no differences were observed among Ischemia Resveratrol and sham groups. The glomerular volumetric density and number of glomeruli of Ischemia were lower than Sham but Ischemia Resveratrol had no difference compared to sham groups. Conclusions Preoperative administration of resveratrol has renoprotective effects, preventing the glomerular number reduction observed in warm ischemia.