Outbreak of pseudomonas aeruginosa causing nosocomial infection in intensive care unit:investigation and strategy

OBJECTIVE To investigate an outbreak of Pseudomonas aeruginosa nosocomial infection in an intensive care unit and find out the cause,the risk factor in order to provide the effective strategy to control the same event of nosocomial infection.METHODS The samples were collected according to the regulation specified by Hospital Degermation Technological Specification.After that,the isolation,culture,and identification as well as susceptibility test of bacteria were carried out according National Clinical Laboratory Operating Instruction.RESULTS The detection rate of P.aeruginosa were 100.0% from sputum aspirator dish samples.66.7% from attendance workers′ hands,50.0% of water tap,40.0% of corrugated pipe of breathing machine,40.0% of cure dish,25.0% of hdoctors′ hands and 16.7% of bedside cupboard in the ICU.The P.aeruginosa isolated from both sputum sample of patient and environment have the same antibiotic sensitivity spectrum which was sensitive to ciprofloxacin,amikacin,and tobramycin but resistive to ceftazidime,cefepime,piperacillin/Tzao and tienam.CONCLUSION The outbreak of P.aeruginosa infection is derived from an case of permanent planting patientltingis due to the inadequacy in breathing machine corrugated tube degermation and personnel′s hands sanitation.In order to prevent the hospital infection,the case isolation,hands sanitation of medical staff,aseptic technique,pipe management of breathing machine should be emphasized.