Studies on Chemical and Biological properties of Bryonia epigaea (Rottler)

To investigate the phytochemical, antimicrobial and antioxidant potentials of Bryonia epigaea (Rottler). The study was performed by using various in-vitro methods such as 1, 1 Diphenyl 2 picryl hydrazyl (DPPH), and Agar well diffusion method for different concentrations of methanolic extracts. Phytochemical constituents of the three extracts (Hexane, Acetone and Methanol) were determined. Total Phenol content was determined by Folin Ciocalteu Method. Yield of extract was determined by caliculating the mass of plant material before minusing the mass of the plant material after the extraction process. Extracts of Bryonia epigaea (Rottler) contained saponins, flavonoids, steroids, tannins, alkaloids, coumarins, phenols and reducing sugars. The antimicrobial activity was moderate. Methanol extract showed good antibacterial activity at 100 mg/ml concentration while antifungal activity against four significant pathogens was moderate. Bryonia epigaea (Rottler) methanol extract exerted significant antioxidant activity and dose dependent effect. The Results showed that the methanol extract showed high amount of antioxidant compounds and exhibit significant antioxidant activity. The antioxidant activity was found to be increased with the concentration of the compound. In the present research work we tried to find out the bioactive properties of ancient medicinal plant Bryonia epigaea (Rottler). The phytochemical, antimicrobial and antioxidant properties have been discussed.   Key words: Bryonia epigaea (Rottler), phytochemical analysis, antimicrobial properties, antioxidant properties.