Influence of single nucleotide polymorphisms in post-bronchiolitis wheezing

INTRODUCTION: a large number of infants present post-bronchiolitis wheezing. It is not clear if genetics factors contribute to it. AIMS: To evaluate the influence of Single Nucleotide Polymorphisms (SNP) in long-term outcomes of patients hospitalized for bronchiolitis. METHODS: All patients hospitalized for bronchiolitis in two winter seasons in 3 tertiary pediatric hospitals were evaluated for epidemiological variables, underwent nasopharyngeal aspirate for detection of virus by PCR technique and blood collection to identify presence of SNPs. Polymorphisms were screened by AccuFill™ System for the OpenArray® Real-Time PCR Platform. Studied SNPs (gene) were: rs4986790 (TLR4), rs4986791 (TLR4), rs1927911 (TLR4), rs898830 (TLR2), rs7656411 (TLR2), rs352162 (TLR9), rs187084 (TLR9), rs1065341 (CCL5), rs2107538 (CCL5), rs2280788 (CCL5), rs2280789 (CCL5), rs10757212 (IFNA5), rs1060826 (NOS2), rs11688 (JUN), rs10735810 (VDR) and rs2228570 (VDR). All patients were followed-up for one year. RESULTS: 85 patients (all CONCLUSIONS: SNPs are associated with worse long-term outcome and maintenance of symptoms in patients hospitalized for bronchiolitis.