Sequence Comparison and Analysis of HA Gene of Four H9N2 Avian Influenza Virus Isolates

[Objective] To determine the HA gene sequences of four H9N2 Avian influenza virus(AIV) strains and carry out comparative analysis so as to understand the difference and variation pattern of each strain from the angle of molecular biology and to know the distribution and epidemic law of H9N2 AIV.[Method] One pair of primers was designed referring to HA gene sequences of H9N2 AIV.The HA genes of A/Chicken/Hebei/WD/98(H9N2;WD98 for short),A/Chicken/Hebei/ZD/04(H9N2;ZD04 for short)),A/Chicken/Beijing/MY/06(H9N2;MY06 for short)),and A/Chicken/Beijing/PG/08(H9N2;PG08 for short)) were amplified,cloned and sequenced.Then the HA gene sequences of these strains were compared with that of 10 H9N2 AIV stains in GenBank.[Result] The ORF of HA genes of the four strains was 1 683 bp in size,encoding 516 amino acids.The HA gene sequences of the four strains,WD98,MY06,PG08,and ZD04,were 82.6%-95.1%,83.0%-99.0%,82.7%-95.5%,and 81.3%-95.7% homologous to that of the 10 H9N2 AIV stains,respectively.And the homology of amino acid was respectively 86.6%-96.3%,86.6%-97.9%,87.0%-97.1%,and 86.9%-97.3%.[Conclusion] The HA gene has greatly high homology among different strains.