Determination of 3 beta-hydroxylated bile acids in human serum and liver tissue.

NAGAKURA, T., SUZUKI, H., MIYAZAKI, Y., OTSUKI, M., GOTO, J., NAMBARA, T. and TOYOTA, T. Determination of 3β-Hydroxylated Bile Acids in Human Serum and Liver Tissue. Tohoku J. Exp. Med., 1989, 159 (2), 165-166-3β, 7α- Dihydroxy-5β-cholanoic acid (3β, 7α-diOH) was detected in serum of 3 patients with intrahepatic cholestasis. 3β, 7β-dihydroxy-5β-cholanoic acid (3β, 7β-diOH) appeared in serum of those patients after treatment with ursodeoxycholic acid (UDC). These bile acids were also detected in only unconjugated fractions of serum of another 7 patients with chronic liver diseases, but not in liver tissue of them. The liver does not seem to from these bile acids itself because they were absent in liver tissue.