P18: The ultrastructure of cerebellar neurons of the Krushinsky-Molodkina strains in dependent of locomotor activity during development hemorrhagic stroke: The role of NO

Background Hemorrhagic stroke (HS) occurs, as a rule, with an increase in blood pressure or hypertension and is caused by ruptured blood vessels to the damage system of self-regulation of cerebral blood flow. Neurological disorders are the characteristic features of stroke. With the development of stroke patients are in a heightened state of spontaneous motor activity (SMA). Activation of blood flow and shear stress on the endothelium of blood vessels at elevated SMA can cause increase in the content of endothelial vascular relaxation factor-nitric oxide (NO) and reduce the pressure. Thus, the increase of NO in the vascular endothelium may be a favorable factor, and the increase of NO in the brain in the development of HS is extremely undesirable. The aim of this work was to study the ultrastructure of rat cerebellar strains K-M under the influence of acoustic stress of their development of hemorrhagic stroke and neurological disorders of the brain. Methods The ultrastructure of the rat cerebellum neuronal network (supragranular layer) was studied by method of the electron-microscopy under HS. The rats were undergone on acoustic stress (AS) with the help of sound (115 db). Results It was compared: (1) the cerebellum structure of the intact rats; (2) rats in the state of HS plus the influence of locomotor activity (LA); (3) rats in state of HS plus the forced rest (FR). In comparison with norm the strong damage of neural network was observed in the case of LA influence: first-edema and some remains neuronal and other cell structures. Relatively the FR variant, it was established that dendrite diameters of granular cell processes weakly have increased possibly account for swelling. Hard damages of ultrastructure were not observed. FR has also induced a decrease of the interbrain blood stroke and besides, FR has decreased the blood stroke sizes of subdural and subarachnoid spaces. Conclusion It was previously shown that inhibitors of inducible and neuronal NO-synthases (aminoguanidine and 7-nitroindazole) significantly reduce mortality, severity of disorders of movement and the development of intracranial hemorrhage caused by audiogenic stress [1] . The studies show that for patients suffering from hemorrhagic stroke, above all, it is advisable to use drugs that reduce the synthesis of NO and create the conditions, approaching physiological sleep. Sources of funding Russian Academy of Sciences. Disclosure Nothing to disclose.