[Correlation study of HPV-16 existential status with Th17/Treg cytokines].

Objective To explore the relationship between the presence of HPV-16 DNA and the expression Treg surface marker Foxp3+, peripheral blood levels of Th17/Treg cell-associated cytokines and explore their roles and significance in cervical cancer progression. Methods Between January 2012 and October 2012 at Shengjing Hospital of China Medical University, a total of 142 HPV16 positive patients were divided into cervical cancer (CC, n=60), cervical intraepithelial neoplasia (CIN,n=65) and control group (n=17).Cervical liquid-based cytological (LBC) samples were collected to detect E2 and E6 genes of HPV type 16 using multiple real-time polymerase chain reaction (PCR).E2/E6 ratio was used to evaluate the physical status of HPV-16 DNA in host cell genome.The SP immunohistochemical method was used to detect the expressions of FOXP3 in cervical lesions.The concentrations of Th17/Treg cell-associated cytokines were detected by enzymelinked immunosorbent assay (ELISA). Results Under the same status of HPV16 DNA in vivo, the levels of Foxp3+, transforming growth factor-β (TGF-β) and interleukin-10 (IL-10) were significantly higher than those of the control group (P 0.05). Conclusion Treg cytokines, HPV16 integration rate and severity of cervical lesions are positively correlated while Th17 cytokines show opposite effects.Th17/Treg cell-associated cytokines may play an important role in the occurrence and development of cervical cancer. Key words: Uterinecervicalneoplasms; T-lymphocytes,regulator; Humanpapillomavirus16; Virusintegration