Magnetic Nano-Sponges for High-Capacity Protein Enrichment and Immobilization.

On the basis of the gel effect of the living RAFT (Reversible Addition-Fragmentation Chain Transfer) polymerization, magnetic nano-sponges with ultrasoft PAA net-like shells are prepared and then used for enriching the entire proteins from real biological samples with ultrahigh capacity. In addition, the immobilized enzyme Cyt c or HRP surprisingly show much higher catalytic activity than free enzymes.