Abstract TP117: Experimental Stroke Induces Chronic Gut Dysbiosis in Male C57BL/6J Mice

Introduction: Recent literature implicates gut epithelia mucosa and intestinal microbiota as important players in post-stroke morbidity and mortality. As most studies have focused on the acute effects of stroke on gut dysbiosis, our study objective was to measure chronic, longitudinal changes in the gut microbiota and intestinal pathology following ischemic stroke. Hypothesis: We hypothesized that mice with experimental ischemic stroke would exhibit chronic gut dysbiosis and intestinal pathology at up to 35 days post-stroke compared to sham controls. Methods: Male C57BL/6J mice were subjected to 60 minutes of transient middle cerebral artery occlusion (tMCAO) or sham-surgery followed by reperfusion and euthanasia at day 35. To determine the long-term effects of tMCAO on gut dysbiosis, fecal boli were collected pre- and post-tMCAO on days 0, 3, 14, and 28. Following DNA extraction, amplification of the V3-V4 region of the 16s rRNA gene was performed via PCR, then library preparation and high-throughput seq...