ANTIBACTERIAL ACTIVITY OF SILVER-EXTRACT NANOPARTICLES SYNTHESIZED FROM THE COMBINATION OF SILVER NANOPARTICLES AND M. CHARANTIA FRUIT EXTRACT

Silver nanoparticles constitute a very promising approach for the development of new antibacterial systems. Nanoparticulate objects can bring significant improvements in the antibacterial activity of this element, through specific effect such as an adsorption at bacterial surfaces. In this study, polyaniline coated AgNPs-extract nanoparticles were investigated for their antibacterial activity. Silver nanoparticles were prepared by the reduction of silver nitrate and NaBH 4 was used as reducing agent. Silver nanoparticles and extracts were mixed thoroughly and then coated by polyaniline. Prepared nanoparticles were characterized by Visual inspection, Ultraviolet-visible spectroscopy (UV), Fourier transform infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) techniques. Antibacterial activities of the synthesized silver nanoparticles were tested against Antibacterial activities of the synthesized Ag-Extract nanoparticles were tested against Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 14028, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 . UV–Vis spectrum of reaction mixture showed strong absorption peak with centering at 400 nm. The FT-IR results imply that Ag-NPs were successfully synthesized and capped with bio-compounds present in Momordica charantia . SEM results revealed that the particle size of these combined nanoparticles was 78.5 to 100 nm in most cases. In most of the cases, Ag-Extract NPs showed better antibacterial activity in compared to our control ciprofloxacin. The result revealed that Ag-extract NPs showed 28±0.68, 8±0.30, 35±0.48, 27±0.35mm zone of inhibition against for S. aureus, S. typhi, E. coli, P. aeruginosa respectively , whereas ciprofloxacin (positive control) showed 23±0.40, 21.40±0.40, 26.18±0.40, 25.32±0.40 mm zone of inhibition for these selected strains. Further studies  should be undertaken to elucidate the exact mechanism  of action  by which  AgNPs-extracts exert  their antimicrobial effect which can  be used in  drug development program for safe health care services. KEYWORDS: Silver nanoparticles, Momordica charantia , Antibacterial activity, Scan Electronic Microscopy (SEM), Zone of inhibition